mouse cd19 micro beads  (Miltenyi Biotec)

Journal: BMB Reports

Article Title: Entinostat, a histone deacetylase inhibitor, increases the population of IL-10 + regulatory B cells to suppress contact hypersensitivity

doi: 10.5483/BMBRep.2021.54.10.092

Figure Lengend Snippet: Entinostat induces IL-10 + B cells from splenic B cells in vitro . (A) The expressions of various isoforms of HDAC isoforms in splenic CD19 + B cells. (B, C) Flow cytometry analysis of IL-10 + splenic B cells. Splenic B cells (3 × 10 6 ) were incubated in 10 μg/ml LPS + PIM with or without entinostat (0-200 nM) for 5 h. Representative dot plot images (B) and frequencies (C) are shown. (D) The histograms show the frequencies of IL-10 + B cells (for LPS 43 h + last PIM 5 h) with or without entinostat (0-200 nM). (E, F) Quantitative analysis of secreted IL-10 and IL-6 in culture supernatant from LPS-stimulated B cells with or without entinostat (0-200 nM) for 48 h. Representative plot images (B) and graphs (A, C, D, E) are the mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, n.s., not significant by Student’s t -test. Ent, entinostat.

Article Snippet: Splenic CD19 + B cells were pre-sorted by the CD19 mAb-micro-beads (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: In Vitro, Flow Cytometry, Incubation